The NeoLNP™ transfection kit (mRNA Universal) represents the next-generation of transfection reagents designed by Scindy Pharmaceutical, utilizing advanced LNP technology specifically for mRNA applications.

Key Features:

• Advanced Ethanol-Free Transfection Vector: Our NeoLNP™ kit built upon the i-Core LNP platform surpasses traditional cationic liposome transfection reagents and sets a new standard in the field of biodegradable ionizable lipid materials by exhibiting rapid metabolization within cells with minimal impact on cellular function and showing robustness under varying cell culture conditions, making it suitable for a wide array of cell types.
• Ease of Use: Encapsulation is carried out within one minute by simply mixing a small amount of sample with NeoLNP™. No need for any type of special equipments.
• Outstanding Delivery Performance: With NeoLNP™ you can easily achieve a transfection rate of over 90%, ensuring the efficient delivery of your mRNA.
• Excellent Safety and Minimal Cytotoxicity: NeoLNP™ is safe for in vitro applications, the cells maintain their viability and health due to the negligible cytotoxicity of NeoLNP™.
• Highly Efficient Gene Translation: NeoLNP™ realizes the efficient expression of target proteins, making it a powerful tool in gene translation studies.

Efficient Somatic Cell Transfection

Using NeoLNP™ mRNA transfection reagent or Lipofectamine 2000, 100 ng of GFP mRNA was separately reverse-transfected into HEK293T, WRL-68, and HFL-1 cells within a 96-well plate. At 24 hours post-transfection, the cells were monitored under a fluorescence microscope. The results demonstrated that NeoLNP™ mRNA transfection reagent had a superior mRNA delivery efficiency in all three cell types compared to Lipofectamine 2000.

Excellent Transfection On Tumor Cells

Using NeoLNP™ mRNA transfection reagent or Lipofectamine 2000, 100 ng of GFP mRNA was separately transfected into A549, MCF7, and Capan-2 cells in a 96-well plate. At 24 hours post-transfection, cells were examined using a fluorescence microscope. The results demonstrated that NeoLNP™ exhibited superior mRNA delivery efficiency in all three types of tumor cells compared to Lipofectamine 2000.

“Zero” Stress on Cells: Very Low Cytotoxicity

Using NeoLNP™, 100 ng of mRNA was seperately reverse-transfected into A549, HFL-1, and TWO3 cells in a 96-well plate. At 24 hours post-transfection, microscopic examination was conducted. The results revealed clear cell margins and homogenous morphologies, with no obvious sign of cell shrinkage or rounding which are indicative of cell death. The cell maintained a good state, further substantiating the remarkably low cytotoxicity associated with NeoLNP™.

Application Scenario